sinaps construct  (Addgene inc)

Journal: eLife

Article Title: Different translation dynamics of β- and γ-actin regulates cell migration

doi: 10.7554/elife.68712

Figure Lengend Snippet: Figure 3. Actin isoform coding sequence affects the overall size and distribution of focal adhesions. Top, representative images of eGFP-actin- transfected cells stained with anti-paxillin to visualize focal adhesions. Total interference reflection fluorescence microscopy (TIRF-M) images of paxillin staining are shown alone (top) and as an overlay with widefield eGFP signals (bottom). Bottom, quantification of focal adhesion size in the four different cell populations, shown as a distribution plot (for all focal adhesions analyzed) and as a scatter plot (for individual cells) in the inset. Scale bar = 10 mm. Figure 3 continued on next page

Article Snippet: Transfected construct (synthetic) pHR-scFv-GCN4sfGFP-GB1-NLS-dWPRE Tanenbaum et al., 2014 Addgene plasmid # 60906 Plasmid containing Superfolder-GFP fused to single-chain variable fragment against GCN4 repeats used to localize nascent peptides from SINAPS constructs Transfected construct (synthetic) pBabe TIR1-9myc Holland et al., 2012 Addgene plasmid # 47328 Plasmid containing the ubiquitin ligase TIR, which targets auxin-induced degrons in nascent peptides from SINAPS constructs Continued on next page Vedula et al. eLife 2021;10:e68712.

Techniques: Sequencing, Transfection, Staining, Fluorescence, Microscopy